RT-PCR vs PCR

1,271 Views | 10 Replies | Last: 3 yr ago by gunan01
rally-cap
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AG
I'm getting married in 2 months, and we are traveling to the Caribbean for our honeymoon. We are required to have a negative PCR test (taken no more than 72 hours before traveling) and our vaccination record before boarding the flight in Miami. We are getting married on Sunday, traveling on Wednesday, but can really only get tested on Monday morning, as Sunday is going to be hard to get to a scheduled appointment in time after the wedding to get tested. I've seen horror stories about people waiting in the airport for a PCR test to come back, only to miss their flight because the test doesn't come back in the promised 24-48 hour window.

I've found some places in DFW that offer a RT-PCR test that gets you results within an hour. I can't find any supporting evidence to explain the differences between the RT-PCR and the PCR tests that I can understand. Would the RT-PCR be accepted the same as a PCR?

Does anyone have any experience with this?
cisgenderedAggie
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No practical difference between PCR and RT-PCR for what you're asking. RT-PCR just means it's amplifying RNA, which is what coronaviruses have.
DFWTLR
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Aren't there dozens of rapid test places all over dfw? Just go first thing monday morning.

We recently went through this and it was a joke, no one verified our tests or even scanned our results. We just showed the airline our printed results that my 4 year old could have produced.
Ranger222
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SARS-COV-2 is a single-stranded RNA virus, meaning viral particles carry RNA as its genetic code rather than DNA.

RT stands for reverse transcriptase, an enzyme that takes the genetic code from an RNA template and generates complementary DNA. Interestingly this enzyme is encoded by viruses themselves and is used to replicate their genomes. Best example is HIV.

To be able to run a quantitative PCR assay, we need DNA as an input. Since SARS-COV-2 is an RNA virus, we must first convert any RNA inside of a sample to DNA. This is what the RT part of RT-PCR assay stands for. Then we can run our PCR detection assay to determine if any and how much viral genetic code is present.

So to specifically answer your question, you actually need the RT-PCR test. Most places offering these services are probably just advertising it as "PCR" when they really mean RT-PCR.

If they are offering 1h RT-PCR services, they must be using one of the Accula detection systems which combine an RT-PCR with a lateral flow strip for hybridization detection...this is not the same as traditional qRT-PCR which will give you a cycle threshold value at the end. I would double check if that would count (have no idea).

In my lab, it takes us at least >3 hours to run a real qRT-PCR assay.... 1 h for the RT reaction, then 2 hours for the qPCR, + the handling time of samples, etc. That's why these tests take so long to turn around. And that's not taking into consideration if you have a backlog of samples in front of yours trying to get on the machine for a result.
rally-cap
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Thank you for your detailed response. This is what I can find -



Both of those only specifically say RT-PCR, so I'm not sure how to answer your question about the non-traditional qRT-PCR...?
tamc93
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rally-cap said:

I'm getting married in 2 months, and we are traveling to the Caribbean for our honeymoon. We are required to have a negative PCR test (taken no more than 72 hours before traveling) and our vaccination record before boarding the flight in Miami. We are getting married on Sunday, traveling on Wednesday, but can really only get tested on Monday morning, as Sunday is going to be hard to get to a scheduled appointment in time after the wedding to get tested. I've seen horror stories about people waiting in the airport for a PCR test to come back, only to miss their flight because the test doesn't come back in the promised 24-48 hour window.

I've found some places in DFW that offer a RT-PCR test that gets you results within an hour. I can't find any supporting evidence to explain the differences between the RT-PCR and the PCR tests that I can understand. Would the RT-PCR be accepted the same as a PCR?

Does anyone have any experience with this?
Just an FYI - you cannot board your DFW flight without the negative test unless it is a separate booking/ticket to Miami.

You should be able to find a lab/urgent care facility etc that can take care of you in plenty of time.

We go to the lab in Austin that does most of the local testing and gets ours within a few hours.
Aston94
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AG
When we travelled to Caribbean in the spring the country required the lab be accredited. So I would verify with the country you are travelling to that the test and lab meet their accreditation requirements.
plain_o_llama
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There was a lot of discussion a few months ago around Ct values. There seemed to be a suggestion that Ct values over some number were unlikely to represent a meaningful infection. Where did all that end up?
Are Ct values reported?

AggieFactor
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This place has had the most dependable turnaround anytime I've need a negative PCR.

https://realtimelab.com/local-testing/travel-testing/
gunan01
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This is a great description. Thanks.
TX05CCHH
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I have a good friend that is stuck in Mexico because he tested positive before trying to come back to the states. He's stuck in a hotel room with security outside for 10 days. So, be careful.
gunan01
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I keep hearing that some of these RT-PCR assays are too sensitive. Rice University recently shut down their campus because of 80+ positive tests in 4000 students. But now they think many of the tests are false positives.

Is there any standardization of these tests to keep them from being too sensitive?
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