agent-maroon said:
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i know more about mRNA than anyone on this board, or on texags in general.
Posted the following on another thread without garnering a response:
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mRNA remains intracellular and doesn't cross the cell membrane. Since antibodies remain extracellular and don't cross the cell membrane, then how are vaccine produced antibodies and viral mRNA ever in contact to initiate an immune system reaction?
Could you offer an explanation?
mRNA is designed to make a protein, not exit the cell. LNPs encapsulate the mRNA and have charge that allows the LNP to pass through the cell membrane and degrade so the mRNA can enter the cytoplasm and attach to the ribosome to make the spike protein. The delivery is not cell type specific in that it cannot distinguish between a myoblast, fibroblast, t-cell etc. It is intramuscular injection so that it can carry throughout the body, but doesn't know which specific cell to transfect, so it transfects any cell it comes in contact. Then it enters that cell via the LNP to make the sequenced protein. So the spike protein will attach to the outer membrane of the cell where it was made. As the virus mutates the spike protein becomes weaker to the new variant because the mRNA wasnt sequenced to create a spike protein to know the new variant. The more mutations of the virus, the weaker the original spike protein becomes. We then have to sequence a new spike protein to create one that can attack the newer variant because the sequence of the spike protein mutates as well. Thats why the vaccine is basically ineffective against more mutated variants as the virus continuallly changes to survive. But thats also why it was more effective against variants like delta. Even though viral sequence mutated, the sequence of the spike protein hadnt, so it still offered a level of protection against delta. Now that the new boosters have been resequenced, it allows for the new spike protein to be created to fight omicron.
mRNA is transiently expressed so once it does its job, it degrades. Also, the other fact about the mRNA is thats its not reversed transcribed, so it doesnt insert into the genome as it doesnt enter the nucleus. Some scientist tried to say it did by using aged Huh7 cells but that study was easily debunked. OTOH, viruses all transcribe into the genome, but mRNA cant unless you sequence it to actuay reverse transcribe, which it doesnt. So the beauty of the vaccine is that it only makes the protein its told to and doesnt get inserted into the genome. The bad part is that we have to continue with injections because its not reverse transcribes so it will never be a part of our dna code like being infected by the virus.
