All the planes took off from the same spot without difficulty except for one plane that took off without flaps. They had trained for the short take off and all the pilots were competent.
Prior to releasing brakes they had 40-50 knots indicated airspeed from wind and carrier motion. In practice, sone takeoffs were made with as little as 60knots indicated.
While it would have been nice to have more takeoff roll (ask any pilot), the main reason for takeoff at the same spot was due to the Hornet's island as they had 6 ft of clearance between it and the right wing tip. There was also an overhanging structure on the island that they needed to pass under. The first two planes took off almost immediately with the second almost stalling so the crews were instructed to adjust their elevator trim to keep them on the deck a little longer.
The pilots were not worried about takeoff, but I think one mentioned that probably would have changed if Doolittles plane didnt make it.